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Addgene

GG-EBNA-MIR302-7guides-PGK-Puro
(Plasmid #201960)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201960 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    GG-EBNA
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIR302-7g-PGK-Puro
  • gRNA/shRNA sequence
    7 gRNAs, see full sequence
  • Species
    H. sapiens (human)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GG-EBNA-MIR302-7guides-PGK-Puro was a gift from Timo Otonkoski (Addgene plasmid # 201960 ; http://n2t.net/addgene:201960 ; RRID:Addgene_201960)

  • For your References section:

    CRISPR activation enables high-fidelity reprogramming into human pluripotent stem cells. Sokka J, Yoshihara M, Kvist J, Laiho L, Warren A, Stadelmann C, Jouhilahti EM, Kilpinen H, Balboa D, Katayama S, Kyttala A, Kere J, Otonkoski T, Weltner J, Trokovic R. Stem Cell Reports. 2022 Feb 8;17(2):413-426. doi: 10.1016/j.stemcr.2021.12.017. Epub 2022 Jan 20. 10.1016/j.stemcr.2021.12.017 PubMed 35063129
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