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pET-His6-en1dFnCas9GFP
(Plasmid #201950)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201950 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-His6-GFP-TEV-LIC
  • Backbone size w/o insert (bp) 6075
  • Total vector size (bp) 11029
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Expression of en1dFnCas9-GFP in bacterial cells
  • Species
    Synthetic
  • Insert Size (bp)
    4944
  • Mutation
    E1369R on FnCas9
  • Promoter T7
  • Tags / Fusion Proteins
    • 6x His (N terminal on backbone)
    • mEGFP (N terminal on backbone)
    • HA (N terminal on insert)
    • NLS (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-His6-en1dFnCas9GFP was a gift from Debojyoti Chakraborty (Addgene plasmid # 201950 ; http://n2t.net/addgene:201950 ; RRID:Addgene_201950)

  • For your References section:

    PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics. Acharya S, Ansari AH, Kumar Das P, Hirano S, Aich M, Rauthan R, Mahato S, Maddileti S, Sarkar S, Kumar M, Phutela R, Gulati S, Rahman A, Goel A, Afzal C, Paul D, Agrawal T, Pulimamidi VK, Jalali S, Nishimasu H, Mariappan I, Nureki O, Maiti S, Chakraborty D. Nat Commun. 2024 Jun 28;15(1):5471. doi: 10.1038/s41467-024-49233-w. 10.1038/s41467-024-49233-w PubMed 38942756
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