pAG416GAL-DENV_NS3-GS-NS2B
(Plasmid
#201940)
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PurposeExpresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201940 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAG416GAL-ccdB
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDENV NS3-GS-NS2B
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Alt nameDengue Virus NS3-GS-NS2B
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Entrez GenePOLY (a.k.a. DV1_gp1, polyprotein gene)
- Promoter GAL1
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer TCAAATGTAATAAAAGTATCAACAAAA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG416GAL-DENV_NS3-GS-NS2B was a gift from Alejandro Chavez (Addgene plasmid # 201940 ; http://n2t.net/addgene:201940 ; RRID:Addgene_201940)
