pRSET mNG-GECO1
(Plasmid
#201933)
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PurposeExpresses mNG-GECO1 indicator in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201933 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSET
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNeonGreenGECO1
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Alt namemNG-GECO1
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Alt namemNG-GECO
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Alt namemNeonGreen Ca2+ indicator
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SpeciesSynthetic
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Insert Size (bp)1416
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Tag
/ Fusion Protein
- HIS tag (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSET mNG-GECO1 was a gift from Robert Campbell (Addgene plasmid # 201933 ; http://n2t.net/addgene:201933 ; RRID:Addgene_201933) -
For your References section:
Bright and High-Performance Genetically Encoded Ca(2+) Indicator Based on mNeonGreen Fluorescent Protein. Zarowny L, Aggarwal A, Rutten VMS, Kolb I, Patel R, Huang HY, Chang YF, Phan T, Kanyo R, Ahrens MB, Allison WT, Podgorski K, Campbell RE. ACS Sens. 2020 Jul 24;5(7):1959-1968. doi: 10.1021/acssensors.0c00279. Epub 2020 Jul 8. 10.1021/acssensors.0c00279 PubMed 32571014