FLAG-Bmp7
(Plasmid
#201909)
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Purposein vitro transcription of RNA encoding FLAG-tagged Bmp7a
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 201909 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
- Backbone size w/o insert (bp) 4000
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namebmp7
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Alt namebmp7a
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1344
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Entrez Genebmp7a (a.k.a. bmp7, fb83f09, wu:fb83f09)
- Promoter SP6
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Tag
/ Fusion Protein
- FLAG tag
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-Bmp7 was a gift from Mary Mullins (Addgene plasmid # 201909 ; http://n2t.net/addgene:201909 ; RRID:Addgene_201909) -
For your References section:
Bone morphogenetic protein heterodimers assemble heteromeric type I receptor complexes to pattern the dorsoventral axis. Little SC, Mullins MC. Nat Cell Biol. 2009 May;11(5):637-43. doi: 10.1038/ncb1870. Epub 2009 Apr 19. 10.1038/ncb1870 PubMed 19377468