acvr1l-VAAA-HA
(Plasmid
#201905)
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Purposein vitro transcription of RNA encoding HA-tagged Acvr1l that contains 4 amino acid substitutions: T186V, S187A, S189A, S191A
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201905 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4163
- Total vector size (bp) 5681
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameacvr1l
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Alt namealk2
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Alt namealk8
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SpeciesD. rerio (zebrafish)
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MutationT186V, S187A, S189A, S191A
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Entrez Geneacvr1l (a.k.a. acvr1, alk2, alk8, alk8sum, etID124543.2, fk50h05, laf, lost-a-fin, mfn, wu:fk50h05)
- Promoter SP6
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
acvr1l-VAAA-HA was a gift from Mary Mullins (Addgene plasmid # 201905 ; http://n2t.net/addgene:201905 ; RRID:Addgene_201905) -
For your References section:
BMP heterodimers signal via distinct type I receptor class functions. Tajer B, Dutko JA, Little SC, Mullins MC. Proc Natl Acad Sci U S A. 2021 Apr 13;118(15):e2017952118. doi: 10.1073/pnas.2017952118. 10.1073/pnas.2017952118 PubMed 33827919