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Addgene

pET3-ARF4-His6(CO)
(Plasmid #201885)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201885 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3C
  • Backbone size w/o insert (bp) 4600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARF4
  • Species
    H. sapiens (human)
  • Mutation
    Codon optimized for bacterial expression
  • Entrez Gene
    ARF4 (a.k.a. ARF2)
  • Tag / Fusion Protein
    • 6xHis (GSGSGSHHHHHH) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET3-ARF4-His6(CO) was a gift from Richard Kahn (Addgene plasmid # 201885 ; http://n2t.net/addgene:201885 ; RRID:Addgene_201885)
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