pMflT-o4-mNG
(Plasmid
#201855)
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PurposeMesoplasma florum oriC plasmid expressing mNeonGreen fluorescent protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201855 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMflT-o4
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature30°C
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Growth Strain(s)EC100pir+
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNeonGreen
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The user should always streak the culture on plate and work with isolated fluorescent colonies. Addgene NGS results found mixed population of nucleotides across Term_apFAB390 and C-terminus of NeonGreen, but plasmid function should not be affected.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMflT-o4-mNG was a gift from Sébastien Rodrigue (Addgene plasmid # 201855 ; http://n2t.net/addgene:201855 ; RRID:Addgene_201855)
Map uploaded by the depositor.
