pB-CAG-FLEX-F14F15-BFP-(mCherry)'
(Plasmid
#201831)
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PurposepiggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201831 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepB-CAG
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 8924
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCAG
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Alt nameCAG promoter
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SpeciesSynthetic
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Insert Size (bp)1699
- Promoter CAG
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameBFP
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Alt namemTagBFP2
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SpeciesSynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.02.23.481706v3 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB-CAG-FLEX-F14F15-BFP-(mCherry)' was a gift from Ian Wickersham (Addgene plasmid # 201831 ; http://n2t.net/addgene:201831 ; RRID:Addgene_201831) -
For your References section:
Third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency. Jin L, Sullivan HA, Zhu M, Lea NE, Lavin TK, Fu X, Matsuyama M, Hou Y, Feng G, Wickersham IR. Cell Rep Methods. 2023 Nov 20;3(11):100644. doi: 10.1016/j.crmeth.2023.100644. 10.1016/j.crmeth.2023.100644 PubMed 37989085