3XFlagNICD1
(Plasmid #20183)

• Depositing Lab: Raphael Kopan
• Publication: Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19.

Backbone

• Vector backbone: p3XFLAG-CMV-7
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Notch 1 Intracellular Domain
• Alt name: NICD1
• Alt name: Notch 1
• Species: M. musculus (mouse)
• Insert Size (bp): 2361
• Mutation: Contains murine Notch 1 Intracellular Domain (Val-1744 to Lys-2531)
• GenBank ID: AAM28905.1 AF508809_1
• Entrez Gene: Notch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3XFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Full length Notch-1 cDNA from Jeffrey S. Nye (Northwestern University School of Medicine, Chicago, IL)
• Articles Citing this Plasmid:
  • 29 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD1 was cloned into XbaI/BamHI sites. NICD1 (Val-1744 to Lys-2531 of full-length mouse N1) was amplified using primers 5XbaN1 (5'-GCCTCTAGAGTGCTGCTGTCCCGCAAGCGC-3'
) and 3BamN1 (5'
-CGGGATCCTTATTTAAATGCCTCTGGAATGTGGG-3
').