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H5_fold-0_Elsa
(Plasmid #201825)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201825 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b(+)
  • Backbone size w/o insert (bp) 5708
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For protein expression, BL21 star is recommended
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H5_fold-0_Elsa
  • Species
    Synthetic
  • Insert Size (bp)
    285
  • Tag / Fusion Protein
    • 6xHis, TEV cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H5_fold-0_Elsa was a gift from Nobuyasu Koga (Addgene plasmid # 201825 ; http://n2t.net/addgene:201825 ; RRID:Addgene_201825)

  • For your References section:

    Design of complicated all-alpha protein structures. Sakuma K, Kobayashi N, Sugiki T, Nagashima T, Fujiwara T, Suzuki K, Kobayashi N, Murata T, Kosugi T, Tatsumi-Koga R, Koga N. Nat Struct Mol Biol. 2024 Feb;31(2):275-282. doi: 10.1038/s41594-023-01147-9. Epub 2024 Jan 4. 10.1038/s41594-023-01147-9 PubMed 38177681
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