pMAH-pnRFP-B30Y
(Plasmid
#201823)
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PurposeExpresses pnRFP-B30Y in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201823 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMAH
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepnRFP-B30Y
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SpeciesSynthetic
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Insert Size (bp)700
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer atttaggtgacactataga (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAH-pnRFP-B30Y was a gift from Huiwang Ai (Addgene plasmid # 201823 ; http://n2t.net/addgene:201823 ; RRID:Addgene_201823) -
For your References section:
Development, Characterization, and Structural Analysis of a Genetically Encoded Red Fluorescent Peroxynitrite Biosensor. Pang Y, Huang M, Fan Y, Yeh HW, Xiong Y, Ng HL, Ai HW. ACS Chem Biol. 2023 Jun 16;18(6):1388-1397. doi: 10.1021/acschembio.3c00139. Epub 2023 May 15. 10.1021/acschembio.3c00139 PubMed 37185019