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pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa)
(Plasmid #201821)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201821 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOTTC901
  • Backbone manufacturer
    NIDA GEVVC
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hChR2(H134R)-V5-ER-LBD (ChRERa)
  • Alt name
    ChRERa
  • Species
    H. sapiens (human); C. reinhardtii
  • Insert Size (bp)
    2000
  • Promoter SYN1

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

3' Cloning Site: NheI (not destroyed), 5' Cloning Site: AscI (not destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa) was a gift from Christopher Richie (Addgene plasmid # 201821 ; http://n2t.net/addgene:201821 ; RRID:Addgene_201821)

  • For your References section:

    Expression of the excitatory opsin ChRERalpha can be traced longitudinally in rat and nonhuman primate brains with PET imaging. Bonaventura J, Boehm MA, Jedema HP, Solis O, Pignatelli M, Song X, Lu H, Richie CT, Zhang S, Gomez JL, Lam S, Morales M, Gharbawie OA, Pomper MG, Stein EA, Bradberry CW, Michaelides M. Sci Transl Med. 2023 Jul 26;15(706):eadd1014. doi: 10.1126/scitranslmed.add1014. Epub 2023 Jul 26. 10.1126/scitranslmed.add1014 PubMed 37494470
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