pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD
(Plasmid
#201818)
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PurposeA plasmid expressing the ligand-binding domain of estrogen receptor with a V5 epitope tag from a CMV promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201818 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameV5-ER-LBD
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
- Promoter CMV-IE
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
3' Cloning Site: NheI (destroyed), 5' Cloning Site: EcoRI (unknown).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD was a gift from Christopher Richie (Addgene plasmid # 201818 ; http://n2t.net/addgene:201818 ; RRID:Addgene_201818) -
For your References section:
Expression of the excitatory opsin ChRERalpha can be traced longitudinally in rat and nonhuman primate brains with PET imaging. Bonaventura J, Boehm MA, Jedema HP, Solis O, Pignatelli M, Song X, Lu H, Richie CT, Zhang S, Gomez JL, Lam S, Morales M, Gharbawie OA, Pomper MG, Stein EA, Bradberry CW, Michaelides M. Sci Transl Med. 2023 Jul 26;15(706):eadd1014. doi: 10.1126/scitranslmed.add1014. Epub 2023 Jul 26. 10.1126/scitranslmed.add1014 PubMed 37494470