pCINeo CD3 delta
(Plasmid
#201814)
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PurposeMammalian Expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 201814 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCINeo
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD3-delta
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SpeciesM. musculus (mouse)
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Insert Size (bp)400
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Mutationnone
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Entrez GeneCd3d (a.k.a. T3d)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCINeo CD3 delta was a gift from Allan Weissman (Addgene plasmid # 201814 ; http://n2t.net/addgene:201814 ; RRID:Addgene_201814) -
For your References section:
The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum. Fang S, Ferrone M, Yang C, Jensen JP, Tiwari S, Weissman AM. Proc Natl Acad Sci U S A. 2001 Dec 4. 98(25):14422-7. 10.1073/pnas.251401598 PubMed 11724934