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Addgene

pCINeo CD3 delta
(Plasmid #201814)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201814 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCINeo
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD3-delta
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    400
  • Mutation
    none
  • Entrez Gene
    Cd3d (a.k.a. T3d)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCINeo CD3 delta was a gift from Allan Weissman (Addgene plasmid # 201814 ; http://n2t.net/addgene:201814 ; RRID:Addgene_201814)
  • For your References section:

    The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum. Fang S, Ferrone M, Yang C, Jensen JP, Tiwari S, Weissman AM. Proc Natl Acad Sci U S A. 2001 Dec 4. 98(25):14422-7. 10.1073/pnas.251401598 PubMed 11724934