UBE2G2 shRNA
(Plasmid
#201813)
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PurposesiRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSUPER
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Vector typeRNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUBE2G2
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gRNA/shRNA sequenceunspecified
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SpeciesH. sapiens (human)
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Entrez GeneUBE2G2 (a.k.a. UBC7)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site Hind III (destroyed during cloning)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
contains bases 391-411 of the coding region
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UBE2G2 shRNA was a gift from Allan Weissman (Addgene plasmid # 201813 ; http://n2t.net/addgene:201813 ; RRID:Addgene_201813) -
For your References section:
The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B, Mariano J, Tsai YC, Chan AH, Cohen M, Weissman AM. Proc Natl Acad Sci U S A. 2006 Jan 10. 103(2):341-6. 10.1073/pnas.0506618103 PubMed 16407162