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Addgene

UBE2G2 shRNA
(Plasmid #201813)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201813 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSUPER
  • Vector type
    RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBE2G2
  • gRNA/shRNA sequence
    unspecified
  • Species
    H. sapiens (human)
  • Entrez Gene
    UBE2G2 (a.k.a. UBC7)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site Hind III (destroyed during cloning)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

contains bases 391-411 of the coding region

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UBE2G2 shRNA was a gift from Allan Weissman (Addgene plasmid # 201813 ; http://n2t.net/addgene:201813 ; RRID:Addgene_201813)
  • For your References section:

    The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B, Mariano J, Tsai YC, Chan AH, Cohen M, Weissman AM. Proc Natl Acad Sci U S A. 2006 Jan 10. 103(2):341-6. 10.1073/pnas.0506618103 PubMed 16407162