pET28a-TG2x-DogTag2
(Plasmid
#201811)
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PurposeBacterial expression of a modified Transglutaminase 2 with a peptide tag for modular ligation directed by SnoopLigase2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201811 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6896
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTG2x
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Alt nameTransglutaminase 2
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SpeciesSynthetic
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Insert Size (bp)1527
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MutationC230S mutation to slow oxidative inactivation of transamidase activity
- Promoter T7
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Tags
/ Fusion Proteins
- DogTag2 (C terminal on insert)
- His10 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-TG2x-DogTag2 was a gift from Mark Howarth (Addgene plasmid # 201811) -
For your References section:
Design and Evolution of Enhanced Peptide-Peptide Ligation for Modular Transglutaminase Assembly. Keeble AH, Wood DP, Howarth M. Bioconjug Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122. 10.1021/acs.bioconjchem.3c00122 PubMed 37289810