pcDNA3.1 RBD-DogTag2-SpyTag
(Plasmid
#201806)
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PurposeMammalian expression of SARS-CoV-2 Receptor Binding Domain with peptide tags for covalent bond formation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6250
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRBD
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Alt nameSARS-CoV-2 receptor binding domain
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SpeciesSynthetic
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Insert Size (bp)822
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
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Tags
/ Fusion Proteins
- Signal peptide (N terminal on insert)
- DogTag2 (C terminal on insert)
- SpyTag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 RBD-DogTag2-SpyTag was a gift from Mark Howarth (Addgene plasmid # 201806) -
For your References section:
Design and Evolution of Enhanced Peptide-Peptide Ligation for Modular Transglutaminase Assembly. Keeble AH, Wood DP, Howarth M. Bioconjug Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122. 10.1021/acs.bioconjchem.3c00122 PubMed 37289810