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pcDNA3.1 HA-MBP-SnoopLigase2 N775Q-KDEL
(Plasmid #201805)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201805 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7050
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SnoopLigase2
  • Species
    Synthetic
  • Insert Size (bp)
    1623
  • Mutation
    N775Q mutation to abolish glycosylation
  • Tags / Fusion Proteins
    • Signal peptide (N terminal on insert)
    • HA tag (N terminal on insert)
    • MBP (N terminal on insert)
    • His6 (N terminal on insert)
    • KDEL (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 HA-MBP-SnoopLigase2 N775Q-KDEL was a gift from Mark Howarth (Addgene plasmid # 201805)

  • For your References section:

    Design and Evolution of Enhanced Peptide-Peptide Ligation for Modular Transglutaminase Assembly. Keeble AH, Wood DP, Howarth M. Bioconjug Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122. 10.1021/acs.bioconjchem.3c00122 PubMed 37289810
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