pENTR4 TfR-sfGFP-SnoopTag2
(Plasmid
#201804)
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PurposeTransferrin receptor (TfR)-based construct to display superfolder green fluorescent protein (sfGFP) linked to SnoopTag2 on the surface of mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201804 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepENTR4
- Backbone size w/o insert (bp) 4766
- Total vector size (bp) 5828
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTfR-sfGFP
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SpeciesSynthetic
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Insert Size (bp)1061
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Tag
/ Fusion Protein
- SnoopTag2 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer BGHF
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR4 TfR-sfGFP-SnoopTag2 was a gift from Mark Howarth (Addgene plasmid # 201804) -
For your References section:
Design and Evolution of Enhanced Peptide-Peptide Ligation for Modular Transglutaminase Assembly. Keeble AH, Wood DP, Howarth M. Bioconjug Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122. 10.1021/acs.bioconjchem.3c00122 PubMed 37289810
