Tensin-2 Y483A
(Plasmid
#201796)
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PurposeExpresses Tensin-2 protein with Y483A mutation in MAC-Tag-C backbone
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201796 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMAC-tag-C
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Backbone manufacturerMarkku Varjosalo (Addgene plasmid # 108077)
- Backbone size w/o insert (bp) 7979
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTensin-2
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SpeciesM. musculus (mouse)
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Mutationmutation Y483A
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GenBank IDNP_705761.2
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Entrez GeneTns2 (a.k.a. C1-ten, Tenc1, nep, nph)
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Tag
/ Fusion Protein
- BirA, HA, strepIII (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Tensin-2 Y483A was a gift from Vesa Hytönen (Addgene plasmid # 201796 ; http://n2t.net/addgene:201796 ; RRID:Addgene_201796) -
For your References section:
Tensin-2 interactomics reveals interaction with GAPDH and a phosphorylation-mediated regulatory role in glycolysis. Turkki P, Chowdhury I, Ohman T, Azizi L, Varjosalo M, Hytonen VP. Sci Rep. 2024 Aug 27;14(1):19862. doi: 10.1038/s41598-024-65787-7. 10.1038/s41598-024-65787-7 PubMed 39191795