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Addgene

pCS-lpp0.2-K64A
(Plasmid #201789)

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Item Catalog # Description Quantity Price (USD)
Plasmid 201789 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCS-lpp0.2-PadR
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PadR
  • Species
    Bacillus subtilis
  • Mutation
    K64A
  • Entrez Gene
    padR (a.k.a. EO946_RS04470, EO946_04470)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS-lpp0.2-K64A was a gift from Yajun Yan (Addgene plasmid # 201789 ; http://n2t.net/addgene:201789 ; RRID:Addgene_201789)

  • For your References section:

    Optimization of a p-Coumaric Acid Biosensor System for Versatile Dynamic Performance. Jiang T, Li C, Yan Y. ACS Synth Biol. 2021 Jan 15;10(1):132-144. doi: 10.1021/acssynbio.0c00500. Epub 2020 Dec 30. 10.1021/acssynbio.0c00500 PubMed 33378169
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