pAAV-U6-AlbEx14-mNG
(Plasmid #201780)

• Purpose: knocks in mNeonGreen into exon 14 of Alb to produce Alb-mNeonGreen fusion protein in mice
• Depositing Lab: Hajime Hirase
• Publication: Vittani et al Fluorescence Imaging of the Brain. Neuromethods, vol 209. Humana, New York, NY.

Backbone

• Vector backbone: pAAV.GFAP.Cre.WPRE.hGH
• Backbone manufacturer: Addgene plasmid #105550
• Backbone size w/o insert (bp): 2876
• Total vector size (bp): 7454
• Vector type: AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Alb gRNA
• Species: M. musculus (mouse)
• Insert Size (bp): 3226
• Entrez Gene: Alb (a.k.a. Alb-1, Alb1, BCL002)
• Promoter: U6

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: Alb_LHA-mNeonGreen-Alb_RHA
• Species: M. musculus (mouse)
• Entrez Gene: Alb (a.k.a. Alb-1, Alb1, BCL002)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pAAV-U6-AlbEx14-mNG.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.07.10.548084v1 for bioRxiv preprint.