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Addgene

pMMRSF17
(Plasmid #20178)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20178 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF-1
  • Backbone manufacturer
    novagen
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C-CrkII nSH3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    225
  • Entrez Gene
    CRK (a.k.a. CRKII, p38)
  • Tags / Fusion Proteins
    • Smt3 (N terminal on backbone)
    • NpuNdelC16 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pAT044 obtained from Dr. Patrick Forrer from University of Zuerich
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene 1998 224 45-52

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMMRSF17 was a gift from Hideo Iwai (Addgene plasmid # 20178 ; http://n2t.net/addgene:20178 ; RRID:Addgene_20178)

  • For your References section:

    Segmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing. Muona M, Aranko AS, Iwai H. Chembiochem. 2008 Dec 15. 9(18):2958-61. 10.1002/cbic.200800604 PubMed 19031436
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