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Addgene

pMMRSF1-16
(Plasmid #20177)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20177 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF-1
  • Backbone manufacturer
    novagen
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    src homology domain 3
  • Alt name
    CRK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    276
  • Entrez Gene
    CRK (a.k.a. CRKII, p38)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • NpuDnaEC16 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMMRSF1-16 was a gift from Hideo Iwai (Addgene plasmid # 20177 ; http://n2t.net/addgene:20177 ; RRID:Addgene_20177)

  • For your References section:

    Segmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing. Muona M, Aranko AS, Iwai H. Chembiochem. 2008 Dec 15. 9(18):2958-61. 10.1002/cbic.200800604 PubMed 19031436
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