pABY-c25
(Plasmid
#201769)
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PurposeExpresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeast
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS305
- Backbone size w/o insert (bp) 6086
- Total vector size (bp) 7313
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNbALFA
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Alt nameALFA nanobody
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SpeciesSynthetic
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Insert Size (bp)1227
- Promoter CYC1
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Tag
/ Fusion Protein
- 40aa Linker, mNeonGreen (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer SK
- 3′ sequencing primer KS (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pABY-c25 was a gift from Saravanan Palani (Addgene plasmid # 201769 ; http://n2t.net/addgene:201769 ; RRID:Addgene_201769) -
For your References section:
ALIBY: ALFA Nanobody-Based Toolkit for Imaging and Biochemistry in Yeast. Akhuli D, Dhar A, Viji AS, Bhojappa B, Palani S. mSphere. 2022 Oct 26;7(5):e0033322. doi: 10.1128/msphere.00333-22. Epub 2022 Oct 3. 10.1128/msphere.00333-22 PubMed 36190134
Map uploaded by the depositor.
