pABY-T3
(Plasmid #201766)

• Purpose: For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast
• Depositing Lab: Saravanan Palani
• Publication: Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi: 10.1128/msphere.00333-22. Epub 2022 Oct 3.

Backbone

• Vector backbone: pFA6a
• Backbone size w/o insert (bp): 3763
• Total vector size (bp): 3802
• Vector type: Yeast Expression
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ALFA tag
• Species: Synthetic
• Insert Size (bp): 39

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: pHybLex-R

Resource Information

• Supplemental Documents:
  • pABY-T3.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry