Skip to main content
Addgene

pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D)
(Plasmid #20174)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20174 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    Gateway destination vector
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D)
  • Alt name
    STIM2, FLJ39527, KIAA1482
  • Species
    H. sapiens (human)
  • Mutation
    3 mutations (K83A,D84N,G86D) in the EF hand of STIM2. YFP inserted between signal peptide(SP)and residues 15-746 of STIM2. (Lab plasmid WS5179)
  • Entrez Gene
    STIM2
  • Tag / Fusion Protein
    • YFP (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attB1 (unknown if destroyed)
  • 3′ cloning site attB2 (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

STIM2 also has P38L, V222I, and E575V, as described in the paper's supplemental material. These mutations do not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D) was a gift from Tobias Meyer (Addgene plasmid # 20174 ; http://n2t.net/addgene:20174 ; RRID:Addgene_20174)

  • For your References section:

    STIM2 is a feedback regulator that stabilizes basal cytosolic and endoplasmic reticulum Ca2+ levels. Brandman O, Liou J, Park WS, Meyer T. Cell. 2007 Dec 28. 131(7):1327-39. 10.1016/j.cell.2007.11.039 PubMed 18160041
Related items:
Commonly requested with: