Skip to main content
Addgene

rNrx1beta(47-299)
(Plasmid #20173)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20173 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pACGP67a
  • Backbone manufacturer
    BD Biosciences
  • Backbone size w/o insert (bp) 10000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neurexin 1 beta
  • Alt name
    Nrx1beta
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    669
  • Mutation
    no splice site
  • GenBank ID
    NM_021767
  • Entrez Gene
    Nrxn1 (a.k.a. Nrxn1b)
  • Tag / Fusion Protein
    • 6X His tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer Polyhedron Forward
  • 3′ sequencing primer Polyhedron Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rNrx1beta(47-299) was a gift from Axel Brunger (Addgene plasmid # 20173 ; http://n2t.net/addgene:20173 ; RRID:Addgene_20173)

  • For your References section:

    Structures of neuroligin-1 and the neuroligin-1/neurexin-1 beta complex reveal specific protein-protein and protein-Ca2+ interactions. Arac D, Boucard AA, Ozkan E, Strop P, Newell E, Sudhof TC, Brunger AT. Neuron. 2007 Dec 20. 56(6):992-1003. 10.1016/j.neuron.2007.12.002 PubMed 18093522
Related items:
Commonly requested with: