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Addgene

pJet1.2-tHe-Tub5
(Plasmid #201727)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201727 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJet1.2
  • Backbone manufacturer
    Thermo Fischer Scientific
  • Backbone size w/o insert (bp) 2974
  • Total vector size (bp) 4379

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tub5
  • Species
    Hypsibius exemplaris
  • Insert Size (bp)
    1392
  • GenBank ID
    OQ282848

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer pJET1.2 Forward Sequencing Primer (5'-CGACTCACTATAGGGAGAGCGGC-3')
  • 3′ sequencing primer pJET1.2 Reverse Sequencing Primer (5'-AAGAACATCGATTTTCCATGGCAG-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJet1.2-tHe-Tub5 was a gift from Stanislav Vinopal (Addgene plasmid # 201727 ; http://n2t.net/addgene:201727 ; RRID:Addgene_201727)

  • For your References section:

    Phylogenetic and functional characterization of water bears (Tardigrada) tubulins. Novotna Floriancicova K, Baltzis A, Smejkal J, Czernekova M, Kaczmarek L, Maly J, Notredame C, Vinopal S. Sci Rep. 2023 Mar 30;13(1):5194. doi: 10.1038/s41598-023-31992-z. 10.1038/s41598-023-31992-z PubMed 36997657
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