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Addgene

rNL1(46-638)
(Plasmid #20172)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20172 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACGP67a
  • Backbone manufacturer
    BD Biosciences
  • Backbone size w/o insert (bp) 10000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neuroligin 1
  • Alt name
    NL1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1695
  • Mutation
    no splice sites
  • GenBank ID
    NM_053868
  • Entrez Gene
    Nlgn1
  • Tag / Fusion Protein
    • C-terminal 6X His tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer Polyhedron Forward
  • 3′ sequencing primer Polyhedron Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rNL1(46-638) was a gift from Axel Brunger (Addgene plasmid # 20172 ; http://n2t.net/addgene:20172 ; RRID:Addgene_20172)

  • For your References section:

    Structures of neuroligin-1 and the neuroligin-1/neurexin-1 beta complex reveal specific protein-protein and protein-Ca2+ interactions. Arac D, Boucard AA, Ozkan E, Strop P, Newell E, Sudhof TC, Brunger AT. Neuron. 2007 Dec 20. 56(6):992-1003. 10.1016/j.neuron.2007.12.002 PubMed 18093522

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