pJet1.2-tHe-Tub1C1
(Plasmid
#201717)
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PurposeTardigrade (Hypsibius exemplaris) alpha-tubulin Tub1C1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201717 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJet1.2
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Backbone manufacturerThermo Fischer Scientific
- Backbone size w/o insert (bp) 2974
- Total vector size (bp) 4526
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTub1C1
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SpeciesHypsibius exemplaris
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Insert Size (bp)1359
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GenBank IDOQ282843
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer pJET1.2 Forward Sequencing Primer (5'-CGACTCACTATAGGGAGAGCGGC-3')
- 3′ sequencing primer pJET1.2 Reverse Sequencing Primer (5'-AAGAACATCGATTTTCCATGGCAG-3') (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJet1.2-tHe-Tub1C1 was a gift from Stanislav Vinopal (Addgene plasmid # 201717 ; http://n2t.net/addgene:201717 ; RRID:Addgene_201717) -
For your References section:
Phylogenetic and functional characterization of water bears (Tardigrada) tubulins. Novotna Floriancicova K, Baltzis A, Smejkal J, Czernekova M, Kaczmarek L, Maly J, Notredame C, Vinopal S. Sci Rep. 2023 Mar 30;13(1):5194. doi: 10.1038/s41598-023-31992-z. 10.1038/s41598-023-31992-z PubMed 36997657