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pmch-mTet1-ZF-CD pc3956
(Plasmid #201709)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201709 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-mcherry-Tet1CD pc2547
  • Backbone manufacturer
    our lab
  • Backbone size w/o insert (bp) 5924
  • Total vector size (bp) 9052
  • Modifications to backbone
    A mcherry-tagged Tet1-ZF-CD fusion (pc3956) was constructed by PCR-amplifying the Tet1-ZF domain including a linker sequence from GFP-Tet1-ZF and inserting the amplicon in the mcherry-Tet1-CD backbone by AsiSI restriction.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tet1-ZF_CD
  • Alt name
    Tet1 Zinc finger + Catalytic domain
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2343
  • Mutation
    Zinc finger domain of Tet1 fused to its catalytic domain
  • GenBank ID
    52463 NM_001253857.2
  • Entrez Gene
    Tet1 (a.k.a. 2510010B09Rik, Cxxc6, D10Ertd17e, LCX, mKIAA1676)
  • Promoter CAG
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AsiSI site (not destroyed)
  • 3′ cloning site AsiSI site (not destroyed)
  • 5′ sequencing primer AGACTAgcgatcgctctacgccgc
  • 3′ sequencing primer TACTCTgcgatcgcaagagccTCCT3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmch-mTet1-ZF-CD pc3956 was a gift from Cristina Cardoso (Addgene plasmid # 201709 ; http://n2t.net/addgene:201709 ; RRID:Addgene_201709)

  • For your References section:

    Isoform-specific and ubiquitination dependent recruitment of Tet1 to replicating heterochromatin modulates methylcytosine oxidation. Arroyo M, Hastert FD, Zhadan A, Schelter F, Zimbelmann S, Rausch C, Ludwig AK, Carell T, Cardoso MC. Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8. 10.1038/s41467-022-32799-8 PubMed 36056023
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