pmch-mTet1-ZF-CD pc3956
(Plasmid #201709)

• Purpose: Expresses of mcherry-tagged mTet1-ZF-CD in mammalian cells
• Depositing Lab: Cristina Cardoso
• Publication: Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8.

Backbone

• Vector backbone: pCAG-mcherry-Tet1CD pc2547
• Backbone manufacturer: our lab
• Backbone size w/o insert (bp): 5924
• Total vector size (bp): 9052
• Modifications to backbone: A mcherry-tagged Tet1-ZF-CD fusion (pc3956) was constructed by PCR-amplifying the Tet1-ZF domain including a linker sequence from GFP-Tet1-ZF and inserting the amplicon in the mcherry-Tet1-CD backbone by AsiSI restriction.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tet1-ZF_CD
• Alt name: Tet1 Zinc finger + Catalytic domain
• Species: M. musculus (mouse)
• Insert Size (bp): 2343
• Mutation: Zinc finger domain of Tet1 fused to its catalytic domain
• GenBank ID: 52463 NM_001253857.2
• Entrez Gene: Tet1 (a.k.a. 2510010B09Rik, Cxxc6, D10Ertd17e, LCX, mKIAA1676)
• Promoter: CAG
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AsiSI site (not destroyed)
• 3′ cloning site: AsiSI site (not destroyed)
• 5′ sequencing primer: AGACTAgcgatcgctctacgccgc
• 3′ sequencing primer: TACTCTgcgatcgcaagagccTCCT3

Resource Information

• Supplemental Documents:
  • pc3956_pCAG-mcherry-Tet1-CxxC-CD.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry