pGFP-mTet1s pc3901
(Plasmid #201700)

• Purpose: Expresses of GFP-tagged mTet1s in mammalian cells
• Depositing Lab: Cristina Cardoso
• Publication: Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8.

Backbone

• Vector backbone: GFP Tet1 full-length (pc2271)
• Backbone size w/o insert (bp): 6977
• Total vector size (bp): 11225
• Modifications to backbone: A GFP-tagged construct encoding the short isoform of Tet1 (pc3901) was generated by amplifying the respective fragment from full-length Tet1 (pc2271) and replacing the full-length Tet1 sequence with the Tet1s sequence by restriction with AsiSI and NotI
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tet1s
• Alt name: Tet1 short isoform
• Species: M. musculus (mouse)
• GenBank ID: NM_001406381.1
• Entrez Gene: Tet1 (a.k.a. 2510010B09Rik, Cxxc6, D10Ertd17e, LCX, mKIAA1676)
• Promoter: CAG
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AsiSI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: ATAAGCAGCGATCGCATGGACTGCAGTAGAC
• 3′ sequencing primer: ATAAGCAGCGGCCGCTTAGACCCAACGATTG

Resource Information

• Supplemental Documents:
  • pc3901_pcag-gfp-tet1s.gb
  • pCAG-GFP-Tet1s (pc3901).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry