pmcherry-mVprBP pc2954
(Plasmid #201699)

• Purpose: Expresses of mcherry-tagged mVprBP in mammalian cells
• Depositing Lab: Cristina Cardoso
• Publication: Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8.

Backbone

• Vector backbone: mCherry Uhrf1 (pc1756)
• Backbone size w/o insert (bp): 9322
• Total vector size (bp): 11534
• Modifications to backbone: Mcherry-tagged VprBP (pc2954) was cloned from murine ESC cDNA by overlap-extension PCR to replace Uhrf1 (pc1756) using AsiSI and NotI restriction enzyme sites.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VprBP
• Alt name: DCAF1
• Species: M. musculus (mouse)
• Insert Size (bp): 4532
• Entrez Gene: Dcaf1 (a.k.a. B930007L02Rik, Vprbp, mKIAA0800)
• Promoter: CAG
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AsiSI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: AAAAGCGATCGCATGACTACAGTAGTGGTAC
• 3′ sequencing primer: AAAAGCGGCCGCATCACTCATTCAGAGATAAG

Resource Information

• Supplemental Documents:
  • pc2954_pcag-ch-mvprbp-ib.gb
  • pc2954_pcag-ch-mvprbp-ib.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry