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CovKCR2_mCherry-pcDNA3.1
(Plasmid #201689)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6910
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CovKCR2
  • Species
    Colponema vietnamica
  • Insert Size (bp)
    765
  • GenBank ID
    OP121640 OP121640
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CovKCR2_mCherry-pcDNA3.1 was a gift from John Spudich (Addgene plasmid # 201689 ; http://n2t.net/addgene:201689 ; RRID:Addgene_201689)

  • For your References section:

    Structural Foundations of Potassium Selectivity in Channelrhodopsins. Govorunova EG, Sineshchekov OA, Brown LS, Bondar AN, Spudich JL. mBio. 2022 Dec 20;13(6):e0303922. doi: 10.1128/mbio.03039-22. Epub 2022 Nov 22. 10.1128/mbio.03039-22 PubMed 36413022
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