pMAH-ER-bapaUGAc
(Plasmid
#201674)
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Purposeboronic acid- and peptide-assisted UDP-GlcNAc sensor in ER
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201674 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.0
- Backbone size w/o insert (bp) 6981
- Total vector size (bp) 7925
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameER-bapaUGAc
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SpeciesSynthetic
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Insert Size (bp)944
- Promoter CMV SP6
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Tags
/ Fusion Proteins
- ER leading sequence (N terminal on insert)
- ER retention sequence (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer SP6: CAT TTA GGT GAC ACT ATA G
- 3′ sequencing primer BGHr: TAG AAG GCA CAG TCG AGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.03.01.530644v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAH-ER-bapaUGAc was a gift from Huiwang Ai (Addgene plasmid # 201674 ; http://n2t.net/addgene:201674 ; RRID:Addgene_201674) -
For your References section:
Genetically Encoded Boronolectin as a Specific Red Fluorescent UDP-GlcNAc Biosensor. Zhang J, Li Z, Pang Y, Fan Y, Ai HW. ACS Sens. 2023 Jul 22. doi: 10.1021/acssensors.3c00409. 10.1021/acssensors.3c00409 PubMed 37480329
