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Addgene

pBad-bapaUGAc
(Plasmid #201673)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201673 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD-hisB
  • Backbone size w/o insert (bp) 4074
  • Total vector size (bp) 4958
  • Modifications to backbone
    NO
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    bapaUGAc
  • Species
    Synthetic
  • Insert Size (bp)
    884
  • Promoter araBAD
  • Tag / Fusion Protein
    • Hisx6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer pBAD F: ATG CCA TAG CAT TTT TAT CC
  • 3′ sequencing primer pBAD R: GAT TTA ATC TGT ATC AGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBad-bapaUGAc was a gift from Huiwang Ai (Addgene plasmid # 201673 ; http://n2t.net/addgene:201673 ; RRID:Addgene_201673)

  • For your References section:

    Genetically Encoded Boronolectin as a Specific Red Fluorescent UDP-GlcNAc Biosensor. Zhang J, Li Z, Pang Y, Fan Y, Ai HW. ACS Sens. 2023 Jul 22. doi: 10.1021/acssensors.3c00409. 10.1021/acssensors.3c00409 PubMed 37480329
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