pBad-bapaUGAc
(Plasmid #201673)

• Purpose: boronic acid- and peptide-assisted UDP-GlcNAc sensor
• Depositing Lab: Huiwang Ai
• Publication: Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acssensors.3c00409.

Backbone

• Vector backbone: pBAD-hisB
• Backbone size w/o insert (bp): 4074
• Total vector size (bp): 4958
• Modifications to backbone: NO
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: bapaUGAc
• Species: Synthetic
• Insert Size (bp): 884
• Promoter: araBAD
• Tag / Fusion Protein:
  • Hisx6 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: pBAD F: ATG CCA TAG CAT TTT TAT CC
• 3′ sequencing primer: pBAD R: GAT TTA ATC TGT ATC AGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.03.01.530644v1 for bioRxiv preprint.