pAL250
(Plasmid
#201672)
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Purpose(Empty Backbone) Contains 250 bp region of Pxyl promoter; insensitive to xylose.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201672 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTA962
- Backbone size (bp) 8322
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Modifications to backboneadded promoter Pxyl
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Vector typeExpression in Haloarchaea
- Promoter Pfdx, Pxyl
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.04.06.535941v3 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAL250 was a gift from Alexandre Bisson (Addgene plasmid # 201672 ; http://n2t.net/addgene:201672 ; RRID:Addgene_201672) -
For your References section:
A sweet new set of inducible and constitutive promoters in Haloferax volcanii. Rados T, Andre K, Cerletti M, Bisson A. Front Microbiol. 2023 Aug 10;14:1204876. doi: 10.3389/fmicb.2023.1204876. eCollection 2023. 10.3389/fmicb.2023.1204876 PubMed 37637112
Map uploaded by the depositor.
