pBS-Squ:mCherry
(Plasmid #20163)

• Depositing Lab: Eric Wieschaus
• Publication: Martin et al Nature. 2008 Nov 23. ():.

Backbone

• Vector backbone: pBluescript
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR
• Alt name: sqh spaghetti squash
• Species: D. melanogaster (fly)
• Insert Size (bp): 3503
• Entrez Gene: sqh (a.k.a. Dmel_CG3595, CG3595, DmMRLC_C, Dmel\CG3595, MLC, MLRC, MRLC, Myo, Myo II, Myo-II, Myo-II RLC, MyoII, Myosin II, NMM, RLC, RLC2, RMLC, Sqh, actomyosin, myo II, myoII, myosin, myosin 2, myosin II, pMyo-II, sgh, spg, squ, ssq)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are several polymorphisms in the 3’UTR of the expression construct and should not affect the function.