pGBKCg
(Plasmid #20162)

• Purpose: (Empty Backbone)
• Depositing Lab: Peter Uetz
• Publication: Stellberger et al Proteome Sci. 2010 . 8():8.

Backbone

• Vector backbone: pGBKT7
• Backbone manufacturer: Clontech
• Backbone size (bp): 9011
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • Gal4 DBD (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest for 20162
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are a few mismatches between depositor's sequence and Addgene's sequence; the mismatches should not affect the plasmid function.