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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20161 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGADT7
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Backbone manufacturerClontech
- Backbone size (bp) 9637
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- Gal4 AD (C terminal on backbone)
- SV40 NLS (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Two mutations are present in the LEU2 sequence: D52E and V69A
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGADCg was a gift from Peter Uetz (Addgene plasmid # 20161 ; http://n2t.net/addgene:20161 ; RRID:Addgene_20161) -
For your References section:
Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome. Stellberger T, Häuser R, Baiker A, Pothineni VR, Haas J, Uetz P. Proteome Sci. 2010 . 8():8. 10.1186/1477-5956-8-8 PubMed 20205919
Map uploaded by the depositor.
