CF-PIPK
(Plasmid
#20157)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20157 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneECFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4733
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCFP-FKBP-PIPK
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Alt namePIPK
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SpeciesM. musculus (mouse)
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MutationC-terminus (amino acids 636-661)truncated, R445E, K446E, S264A
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GenBank IDNM_008844
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Entrez GenePip5k1c (a.k.a. AI115456, AI835305, Pip5kIgamma)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CF-PIPK was a gift from Tobias Meyer (Addgene plasmid # 20157 ; http://n2t.net/addgene:20157 ; RRID:Addgene_20157) -
For your References section:
Rapid chemically induced changes of PtdIns(4,5)P2 gate KCNQ ion channels. Suh BC, Inoue T, Meyer T, Hille B. Science. 2006 Dec 1. 314(5804):1454-7. 10.1126/science.1131163 PubMed 16990515