EGFP in pDBEcR-pIND(SP1)-W/Bla
(Plasmid #201558)

• Purpose: Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.
• Depositing Lab: Randy Poon
• Publication: Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754. doi: 10.1242/jcs.260754. Epub 2023 Apr 19.

Backbone

• Vector backbone: pDBEcR- pIND(SP1)/Bla
• Total vector size (bp): 8657
• Vector type: Mammalian Expression, Unspecified ; sleeping beauty transposon
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DBEcR and EGFP
• Species: a hybrid Drosophila/Bombyx ecdysone receptor
• Promoter: EF1a and pIND(SP1)
• Tags / Fusion Proteins:
  • DBEcR-myc (C terminal on insert)
  • FLAG-EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: MfeI (destroyed during cloning)
• 5′ sequencing primer: 5'-AAGAATTCTCTTATCGGTAATCAACCT-3'
• 3′ sequencing primer: 5'-TAGCTCTCAATTGCGACGGTATCG-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry