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p202-Syt1-C2A-I248V
(Plasmid #201554)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p202
  • Backbone size w/o insert (bp) 6442
  • Total vector size (bp) 6838
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Synaptotagmin-1 C2A domain (partial)
  • Alt name
    SYT1
  • Species
    Synthetic; Octopus bimaculoides
  • Insert Size (bp)
    396
  • Mutation
    changed Isoleucine 248 to Valine (numbering based off native gene), codon optimized for E. coli
  • Promoter RBS

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTTATTTTCAAGGTGATATTACTCATATGGTG
  • 3′ sequencing primer TCAGTGGTGGTGGTGGTGGTGCTCGAGttac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p202-Syt1-C2A-I248V was a gift from Joshua Rosenthal (Addgene plasmid # 201554 ; http://n2t.net/addgene:201554 ; RRID:Addgene_201554)

  • For your References section:

    Temperature-dependent RNA editing in octopus extensively recodes the neural proteome. Birk MA, Liscovitch-Brauer N, Dominguez MJ, McNeme S, Yue Y, Hoff JD, Twersky I, Verhey KJ, Sutton RB, Eisenberg E, Rosenthal JJC. Cell. 2023 Jun 8;186(12):2544-2555.e13. doi: 10.1016/j.cell.2023.05.004. Epub 2023 Jun 8. 10.1016/j.cell.2023.05.004 PubMed 37295402
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