pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3
(Plasmid
#201534)
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PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201534 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJUMP24-T24
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsLow copy in Pseudomonas
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameilvA-relE_RBS3_CB3
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SpeciesSynthetic; E. coli
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MutationrhaS W190G
- Promoter PrhaBAD (rhamnose)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3 was a gift from Dan Park (Addgene plasmid # 201534 ; http://n2t.net/addgene:201534 ; RRID:Addgene_201534) -
For your References section:
Prolonging genetic circuit stability through adaptive evolution of overlapping genes. Chlebek JL, Leonard SP, Kang-Yun C, Yung MC, Ricci DP, Jiao Y, Park DM. Nucleic Acids Res. 2023 Jun 1:gkad484. doi: 10.1093/nar/gkad484. 10.1093/nar/gkad484 PubMed 37260076
