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Addgene

YF-Rac1(DN)
(Plasmid #20151)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20151 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EYFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFP-FKBP-Rac1(DN)
  • Alt name
    Rac1
  • Species
    H. sapiens (human)
  • Mutation
    dominant negative mutation
  • Entrez Gene
    RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YF-Rac1(DN) was a gift from Tobias Meyer (Addgene plasmid # 20151 ; http://n2t.net/addgene:20151 ; RRID:Addgene_20151)

  • For your References section:

    An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways. Inoue T, Heo WD, Grimley JS, Wandless TJ, Meyer T. Nat Methods. 2005 Jun . 2(6):415-8. 10.1038/nmeth763 PubMed 15908919
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