ALFA-bArr2
(Plasmid #201497)

• Purpose: Expression of the beta-arrestin2 containing a N-terminal ALFA tag.
• Depositing Lab: Patrick Giguère
• Publication: Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10.1016/j.jbc.2023.105107.

Backbone

• Vector backbone: pcDNA3.1+
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ALFA-bArr2
• Species: H. sapiens (human)
• Insert Size (bp): 1272
• Entrez Gene: ARRB2 (a.k.a. ARB2, ARR2, BARR2)
• Promoter: CMV
• Tag / Fusion Protein:
  • ALFA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: Bgh-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: H. sapiens (human)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry