bArr1-V5
(Plasmid #201492)

• Purpose: Expression of the beta-arrestin1 containing a C-terminal V5 tag.
• Depositing Lab: Patrick Giguère
• Publication: Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10.1016/j.jbc.2023.105107.

Backbone

• Vector backbone: pLX317
• Backbone manufacturer: Broad Institute
• Backbone size w/o insert (bp): 10065
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: bArr1-V5
• Species: H. sapiens (human)
• Insert Size (bp): 1332
• Entrez Gene: ARRB1 (a.k.a. ARB1, ARR1)
• Promoter: EF1a
• Tag / Fusion Protein:
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EF1F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: H. sapiens (human)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry