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Addgene

pRSET-His-APEX2
(Plasmid #201471)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201471 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSETc
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2814
  • Total vector size (bp) 3561
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For protein expression, the plasmid has to be expressed in T7 Express lysY Competent E. coli (NEB C3010I) or equivalent
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    APEX2
  • Species
    Soybean
  • Insert Size (bp)
    747
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    APEX2 was amplified from Addgene plasmid #49386, a kind gift from Dr. Alice Ting, to generate this plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.03.26.534250 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET-His-APEX2 was a gift from Yuh-Nung Jan (Addgene plasmid # 201471 ; http://n2t.net/addgene:201471 ; RRID:Addgene_201471)

  • For your References section:

    SLAPSHOT reveals rapid dynamics of extracellularly exposed proteome in response to calcium-activated plasma membrane phospholipid scrambling. Tuomivaara ST, Teo CF, Jan YN, Wiita AP, Jan LY. Commun Biol. 2024 Aug 29;7(1):1060. doi: 10.1038/s42003-024-06729-x. 10.1038/s42003-024-06729-x PubMed 39210032
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